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Coherence Controlled Holographic Microscope with the digital optics
Vavřinová, Jana ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The Digital Micromirror Device (DMD) technology has been developed especially for Digital Light Processing projectors, which allow the image projection. After this succesful implementation, and thanks to the commercial availibility and low initial cost of the DMD chip, a wide range of other applications became possible. Besides, it may be used in microscopy as a spatial light modulator. For example in Coherence-Controlled Holographic Microscope (CCHM) that finds its use especially for imaging and measurement of live-cell dynamic processes. The DMD chip placed in the illumination part of CCHM allows for broadening the application possibilities. Namely it could be different illumination mode experiments or tomographic applications. The master's thesis deals with the optical design of CCHM with digital optics, i. e. DMD chip. The selection of optical elements for CCHM, the experimental verification of the imaging setup and the process of designing the illumination part are described in detail. In the end, the analysis of different designs for illumination setup with the digital optics in object arm is carried out and the results are compared.
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Confocal module for the Coherence Controlled Holographic Microscope
Kubátová, Eva ; Kozubek, Michal (referee) ; Dostál, Zbyněk (advisor)
The Coherence Controlled Holographic Microscope (CCHM) was developed at BUT Brno for a quantitative phase imaging of living cells. Nowadays it ocurres that its imaging properties are enhanced by the use of additional modules. In the present the microscope is equipped with the epifluorescence module, which allows observation of fluorescently marked living cells. This thesis is going to follow up on the development of this module and is going to extend its options by confocal imaging. The disadvantage of current multi-channel confocal microscopes is a mechanical rotation of the Nipkow discs, which causes undesired mechanical vibrations. That is why in this thesis it is replaced by Digital Micromirror Device. With its use was developed optical system of the whole confocal model, whose correct funcion was simulated in optical CAD. The experimentally verified prototype serves to test the imaging properties. On this basis is designed an application idea of the fluorescence confocal module, which will be possible to connect to the CCHM microscope.
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Stereolithographic printer for producing a cell culture device
Gricová, Monika ; Vacula, Daniel (referee) ; Dostál, Zbyněk (advisor)
Stereolithography printing is one of the most popular 3D printing technologies. This printers use a UV light source for photopolymer curing and can be used for a wide range of applications with high precision and excellent print quality. Commercially available printers do not allow the modifications of the optical and mechanical parameters of the instrument. For this reason, a DLP printer has been designed to allow the optical system modifications and thus changing the printing field and resolution. Another advantage is the possibility to modify already designed mechanical parts. The DLP printer has been designed and tested. The recommended printer parameters settings are listed, which are based on the performed experiments.
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Confocal module for the Coherence Controlled Holographic Microscope
Kubátová, Eva ; Kozubek, Michal (referee) ; Dostál, Zbyněk (advisor)
The Coherence Controlled Holographic Microscope (CCHM) was developed at BUT Brno for a quantitative phase imaging of living cells. Nowadays it ocurres that its imaging properties are enhanced by the use of additional modules. In the present the microscope is equipped with the epifluorescence module, which allows observation of fluorescently marked living cells. This thesis is going to follow up on the development of this module and is going to extend its options by confocal imaging. The disadvantage of current multi-channel confocal microscopes is a mechanical rotation of the Nipkow discs, which causes undesired mechanical vibrations. That is why in this thesis it is replaced by Digital Micromirror Device. With its use was developed optical system of the whole confocal model, whose correct funcion was simulated in optical CAD. The experimentally verified prototype serves to test the imaging properties. On this basis is designed an application idea of the fluorescence confocal module, which will be possible to connect to the CCHM microscope.
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|
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Stereolithographic printer for producing a cell culture device
Gricová, Monika ; Vacula, Daniel (referee) ; Dostál, Zbyněk (advisor)
Stereolithography printing is one of the most popular 3D printing technologies. This printers use a UV light source for photopolymer curing and can be used for a wide range of applications with high precision and excellent print quality. Commercially available printers do not allow the modifications of the optical and mechanical parameters of the instrument. For this reason, a DLP printer has been designed to allow the optical system modifications and thus changing the printing field and resolution. Another advantage is the possibility to modify already designed mechanical parts. The DLP printer has been designed and tested. The recommended printer parameters settings are listed, which are based on the performed experiments.
|
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Coherence Controlled Holographic Microscope with the digital optics
Vavřinová, Jana ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The Digital Micromirror Device (DMD) technology has been developed especially for Digital Light Processing projectors, which allow the image projection. After this succesful implementation, and thanks to the commercial availibility and low initial cost of the DMD chip, a wide range of other applications became possible. Besides, it may be used in microscopy as a spatial light modulator. For example in Coherence-Controlled Holographic Microscope (CCHM) that finds its use especially for imaging and measurement of live-cell dynamic processes. The DMD chip placed in the illumination part of CCHM allows for broadening the application possibilities. Namely it could be different illumination mode experiments or tomographic applications. The master's thesis deals with the optical design of CCHM with digital optics, i. e. DMD chip. The selection of optical elements for CCHM, the experimental verification of the imaging setup and the process of designing the illumination part are described in detail. In the end, the analysis of different designs for illumination setup with the digital optics in object arm is carried out and the results are compared.
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